Research Assistant I - Genetics

Summary

At the Guarnerio lab, we're dedicated to unraveling the complex molecular mechanisms used by tumor cells to create a pro-tumorigenic tumor microenvironment (TME). We utilize cutting-edge technologies like single-cell RNA sequencing and spatial transcriptomics to study both mouse and human tissues. Our research is deeply rooted in translational science, with the goal of developing innovative cancer therapies that reprogram tumor cells and the TME.

Our recent publications include:

• Study of the MIF/CD74 axis in pro-tumorigenic macrophages (Tessaro et al., Cell Reports, 2022)
• Identification of anti-inflammatory, tumor-promoting circular RNAs (Piras et al., Nature Communications, 2022)
• Characterization of glyCAFs and T cell exclusion from the tumor mass (Broz et al., Nature Communications, 2024)

Function:

We are looking to recruit a Research Assistant I to help achieve our goals. The primary responsibility is to perform technical laboratory duties and carry out one of these exciting projects:

• Cancer-Associated Fibroblasts (CAF): Investigate their role in promoting tumor growth and immune exclusion.
• Circular RNAs: Explore novel mechanisms of immune exclusion and evasion.
• Sarcoma Stem/Progenitor Cells: Identify new tumor vulnerabilities and therapeutic targets.

Key Functions:

• Organize laboratory supplies and perform other lab duties as assigned, including but not limited to preparing sterile media, buffers, and stock solutions.
• Organize and execute experiments in a dynamic environment.
• Execute experiments and critically analyze results under the supervision of the PI or other lab members. Follow lab protocols and Standard Operating Procedures (SOPs). Process and evaluate data obtained in accordance with requested methods. Maintain accurate and detailed records of experimental data and protocols.
• Present data at lab meetings to contribute to scientific discussions.
• Assist in drafting study protocols under the guidance of the PI.
• Learn new lab techniques and gain expertise in cancer biology.
• Other duties as assigned.