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Sr Research Assistant - Endocrine Neoplasia & HD-Rsch

Endocrine Neoplasia & HD-Rsch 600703
127128 Requisition #
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The primary purpose of the Senior Research Assistant position is to provide supervision, assistance, and special training to research assistants and postdoctoral fellows in a research environment. The person is also responsible for a series of experiments designed to supply specific information as part of the larger basic research projects. The position contributes to the development of improved techniques, production methods, and/or procedures. Conducts extensive research in the field of molecular technology.

a. Oversee all aspects of the functioning of a basic research laboratory

Must have a comprehensive knowledge base of technical and procedural aspects of laboratory tests with an emphasis on accuracy, precision, normal ranges, and correlation of laboratory test results.

b. Perform principal laboratory techniques

Must be able to perform most principal techniques associated with recombinant DNA work: DNA/RNA isolation, PCR, plasmid cloning, restriction analysis, sequence analysis, bacterial manipulation, and transformation.

Must be able to perform RNAi approaches (including lentiviral approaches) for downregulation of gene expression in cultured cells.

Must be able to perform most principal techniques associated with protein work: protein isolation from tissues and cultured cells, measure protein concentration, perform immunoprecipitation, ELISA, protein gels, and protein blots. Fix mouse tissues/organs for histology or immunohistochemistry/immunofluorescence.

Must be able to assesses gene expression by qRT-PCR, western blotting (LiCor and chemiluminescence), immunocytochemistry, immunohistochemistry, immunofluorescence and reporter gene assays.

Must be able to detect mutational changes of genes by performing functional assays to analyze the impact of the mutation on the function of the gene product.

Must be able to maintain cells in culture as well as perform transient and stable transfections.

c. Mouse work

Must be able to breed transgenic mice and genotype the progeny. This involves tail or ear snips.

Must be able to inoculate cancer cells into mice (subcutaneous and intravenous).

Must be able to monitor tumor growth and assess maximum acceptable tumor burden according to IACUC/NIH regulations.

Must be able to euthanize mice according to IACUC/NIH regulations.

Must understand mouse anatomy and be able to dissect subcutaneous tumors and internal organs.

d. Perform and optimize experiments

Must be willing to learn how to perform and optimize techniques used for characterization of gene expression and gene knockouts. This include:

1) learn and optimize sample RNA for qRT-PCR/RNA-Seq/NGS, and design of qRT-PCR primers

2) learn and optimize protein sample and antibody concentration to perform immunoprecipitation, western blotting, and ELISA assays;

3) learn and optimize cell/tissue fixation protocols, buffers, and antibody host/concentration for immunocytochemistry, immunohistochemistry, and immunofluorescence on cells and tissues (paraffin and OCT embedded samples)

4) learn and optimize methods used for the assessment of tumorigenesis, such as measurement of cell growth in soft agar or Matrigel, cell proliferation rate, cell migration, and cell invasion in Boyden chambers

5) learn and optimize patient’s and mice gene mutational analysis by isolating genomic DNA from blood or tumors, doing PCR and analyzing sequencing results.

a. Performance and supervision of cell culture maintenance, microscopy, ultracentrifugation, spectrophotometry, and lyophilizer.
b. Must be able to assess proper functioning of related laboratory equipment, plan for and schedule maintenance when necessary. Equipment includes centrifuges, spectrophotometers, infrared scanners (LiCor), incubators, etc.
c. Must have good manual dexterity for repetitive grasping, digital manipulation and eye-hand coordination for the manipulation of pipettes, test tubes and other laboratory devices, some of which may be used in a darkroom environment.

a. Responsible for acquiring continuing education in the various technologies involved in and beyond the scope of the laboratory research by researching, reading and communicating with industry and other research laboratory personnel throughout the country and within the institution
b. Researches the literature regularly


Required: Bachelor's degree in one of the natural sciences or related field. Three years of experience in scientific or experimental research work. With preferred degree, one year of required experience. 

It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html

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